Composite

Part:BBa_K3040001

Designed by: TSAI, CHIA-TSEN   Group: iGEM19_NTHU_Taiwan   (2019-10-18)


Lipase A1 (Psychrobacter sp 7195.) -Histag

Lipase A1 from Antarctic deep-sea bacterium, Psychrobacter sp 7195., can catalyze the hydrolysis of triacylglycerols to glycerol and monoacyl-glycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A1 reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drops. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1186


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